What is CheckMate Infidelity Test Kit?

The CheckMate Infidelity Test Kit is a semen detection kit designed for home use (fast and easy result to determine cheating spouse attitude).  It was introduced in the market in 1999 and was granted US Patent 6,764,856 on July 4, 2004.  The CheckMate brand was originally manufactured by Evergreen Industries LLC.  Medimpex United Inc. took over in 2010 through its affiliate CheckMate Test LLC.

The main proposition of CheckMate is that it is able to provide a means for discovering infidelity of a cheating wives and /or husbands by confirming the presence of semen on personal articles such as underwear and panty liners.  The choice of the item to be tested is usually co-related with highly suspect activities such as frequent and extended out-of-town business activities and late-night-outs.  Its main marketing thrust is focused on offered convenience and credible results, claims that are undergoing close scrutiny by industry experts.

1.  Developments in Semen Detection
2.  Types of Semen Detection Tests
3.  Formulation and Method
4.  Uses
5.  Bibliography

Developments in Semen Detection

As early as 1813, man has been trying to study semen stains through the use of the microscope.  The acknowledged founder of the science of toxicology, Mathieu Bonaventure Orfila was credited for making the first attempt towards this end.  However, it was only in 1839 that HL Bayard published the first reliable procedures for microscopic detection of sperm.

The use of a reagent for the creation of a crystalline reaction for semen was developed by A. Florence of Lyons in 1896.  A method of fixing and staining semen for inspection under a microscope was developed by W. Whitney a year after.  Frank Lundquist provided his contribution through the development of the acid phosphatase (AP) test in 1945.  The publication in 1955 of the Measurement of Acid Phosphatase Activity to Identify Seminal Stains was provided by Hazen.

Acid phosphatase is an enzyme found throughout the body but is primarily found in the prostate gland.  This test is widely used in identifying stains of seminal origin.  It consists of lifting a suspect stain with a solution which is then reacted with another substance to form a colored product.

A protein in the seminal fluid referred to as gamma-seminoprotein was first discussed in 1971 by M. Hara.  Not long after, Li and Beling isolated a protein called E1.  The same protein was described in detail in 1978 by Sensabaugh and named it P30.  Purified by Wang in 1979, it was eventually called the Prostate Specific Antigen (PSA).  Quantitative measurement of PSA in the blood was done by Papidero in 1980 while its clinical use as a marker of prostate cancer was initiated by Stamey.

Both methods have been widely used in aid of detection of certain conditions such as prostate cancer and investigations such as rape cases.  These tests have also formed the basis of various semen detection tests used in relation to proving spouse infidelity by confirming the presence of semen in suspect articles.

Semen Detection Tests

Semen detection has hinged itself on three (3) main types of tests including Microscopic Inspection, Acid Phosphatase, and Prostate Specific Antigen.  Microscopic Examination is essentially done to determine the presence of spermatozoa under specific magnification.  It presents certain limitations on semen sample stains from vasectomized men.

The relevance of finding acid phosphatase in a subject stain is the presence of an enzyme that is only found in high concentration in the prostate gland.  Its presence gives rise to a high probability that the subject stain is semen as a direct result of any sexual activity.  The relation of the presence of semen with other circumstances such as the absence of any sexual activity between partners is the main argument in which infidelity is supposed to be confirmed.

Prostate Specific Antigen is one of the major proteins in seminal fluid.  Due to its high amount of concentration, forensic science has found it very useful.  Various semen-detection kits are based on this test.

The kit is designed for use in suspect stains.  The stain is wetted by a transfer solution which is then blotted by absorbent paper to lift and transfer the biological properties of the stain to the paper.  The testing solution is dropped on the paper when it has dried out.  Results are premised on the paper’s change of color.  A purple color obtained within fifteen (15) seconds of application of the solution is considered a positive or affirmative result.  The test is considered negative otherwise.


As a consumer product, CheckMate is solely offered to detect and confirm the presence of semen.  It is not a product intended to prove ownership of semen which is only possible through DNA testing.  Its main market consists of home consumers.

Semen detection test kits have managed to create its market niche due to two main factors – the high rate of infidelity and the desire to keep private, matters pertaining to infidelity.  Home tests provide the needed privacy and even the swiftness of obtaining results.  The question as to whether or not accuracy of results is at par with laboratory-performed tests remains.

Results obtained from CheckMate testing are not admissible in court proceedings pending its ability to overcome its category as a presumptive test.  Private investigators using the kit in their line of work are specifically aware of such limitations.  Industry experts recommend the co-relation of results obtained from the use of any semen detection kit with other tests and procedures.


Infidelity Test Kit to Bust a Cheating Spouse http://www.checkmatetest.com

CheckMate Infidelity Test Kit for Semen-Sperm Stain Detection http://www.getcheckmate.com

Semen Testing http://www.privateinvestigator-info.org

Semen-Sperm http://www.enotes.com/forensic-science

For more information, please visit this articles web page.

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